Dr. A.K. Pateriya

Senior Scientist

M.Sc. Ph.D., Animal Biotechnology

Email : atul[dot]pateriya[at]icar[dot]gov[dot]in, aatulpateriya[at]gmail[dot]com

Diagnostic kits released and under commercialization / Mobile App.

  1. NIHSAD_Avian Influenza Antibody Detection ELISA kit (https://krishi.icar.gov.in/Technology/technologyShowCaseReport.jsp?id=201538127945396)
  2. MULTIPLEX REAL TIME RT-PCR KIT FOR AVIAN INFLUENZA A VIRUS TYPING AND H5 AND H9 SUBTYPING (https://krishi.icar.gov.in/Technology/technologyShowCaseReport.jsp?id=201596626809418)
  3. MULTIPLEX REAL TIME RT-PCR KIT FOR AVIAN INFLUENZA A VIRUS TYPING AND H5N1 SUBTYPING (https://krishi.icar.gov.in/Technology/technologyShowCaseReport.jsp?id=201596857548890)
  4. Mobile App “Bird flu se Suraksha” developed with technical team of NIHSAD - Mobile App

Technology developed and published

  1. Vaccine: Reverse genetics based rgH5N2 vaccine for H5N1 avian influenza virus in chicken.
  2. Rapid visual diagnostic assay for influenza A viruses using gold nanoparticles and peptide nucleic acid.
  3. Molecular beacon probe-based real-time RT-PCR assay for diagnosis of Crimean-Congo hemorrhagic fever.
  4. Development of single-chain Fv against the nucleoprotein of type A influenza virus and its use in ELISA.
  5. Development of real-time RT-PCR assay for detection and quantification of Chikungunya.
  6. Development of real-time RT-PCR assay for detection and quantification of Chikungunya and Japanese encephalitis virus (JEV)

Disease Diagnosis/Outbreak Investigation

  1. Member of the team at ICAR-NIHSAD (formerly known as HSADL) first time ever diagnosed and isolated the avian influenza infection in domestic birds in India.
  2. Member of the team for the COVID-19 Diagnosis at NIHSAD, Bhopal.
  3. Presently looking after the diagnosis of avian influenza in imported and exported poultry and poultry products in India.

Gene Bank Submission: Genome sequencing and characterization (Total - 95 genomes submitted)

  1. SARS CoV-2 Whole Genome (GISAID) - 09
  2. Avian Influenza A Virus -30
  3. Crimean-Congo hemorrhagic fever virus - 01
  4. Bovine viral diarrhea virus 3 - 38
  5. Bovine viral diarrhea virus 1 - 02
  6. Ovine gammaherpesvirus 2 - 13
  7. Porcine parvovirus - 01
  8. Porcine circovirus – 01

Training Organized

  1. International training conducted at NIHSAD - 03
  2. National Training Program conducted at NIHSAD – 12
















Journals

  1. Kumar N, Bhatia S, Pateriya AK, Sood R, Nagarajan S, Murugkar HV, Kumar S, Singh P, Singh VP. Label-free peptide nucleic acid biosensor for visual detection of multiple strains of influenza A virus suitable for field applications. Anal Chim Acta. 2020 Jan 6;1093:123-130. PMID: 31735205.
  2. Kumar N, Bhatia S, Sood R, Pateriya AK, and Malik YPS. (2019). Immunoinformatics: where immunology meets bioinformatics. Journal of Immunology and Immunopathology, 21(2): 55-67.http://krishi.icar.gov.in/jspui/handle/123456789/29382.
  3. Kumar N, Kulkarni DD, Lee B, Kaushik R, Bhatia S, Sood R, Pateriya AK, Bhat S, Singh VP. Evolution of Codon Usage Bias in Henipaviruses Is Governed by Natural Selection and Is Host-Specific. Viruses. 2018 Nov 1;10(11):604. doi: 10.3390/v10110604. PMID: 30388838; PMCID: PMC6266499.3. Sood R, Kumar N, Bhatia S, Chanu KV, Gupta CL, Pateriya AK, Mishra A, Khandia R, Mawale N, Singh VP. Neuraminidase inhibitors susceptibility profiles of highly pathogenic influenza A (H5N1) viruses isolated from avian species in India (2006-2015). Antiviral Res. 2018 Oct;158:143-146. PMID: 30125616.
  4. Sood R, Kumar N, Bhatia S, Chanu KV, Gupta CL, Pateriya AK, Mishra A, Khandia R, Mawale N, Singh VP. Neuraminidase inhibitors susceptibility profiles of highly pathogenic influenza A (H5N1) viruses isolated from avian species in India (2006-2015). Antiviral Res. 2018 Oct;158:143-146. PMID: 30125616.
  5. Ghoke SS, Sood R, Kumar N, Pateriya AK, Bhatia S, Mishra A, Dixit R, Singh VK, Desai DN, Kulkarni DD, Dimri U, Singh VP. Evaluation of antiviral activity of Ocimum sanctum and Acacia arabica leaves extracts against H9N2 virus using embryonated chicken egg model. BMC Complement Altern Med. 2018 Jun 5;18(1):174. PMID: 29866088; PMCID: PMC5987647.
  6. Khandia R, Pattnaik B, Rajukumar K, Pateriya A, Bhatia S, Murugkar H, Prakash A, Pradhan HK, Dhama K, Munjal A, Joshi SK. Anti-proliferative role of recombinant lethal toxin of Bacillus anthracis on primary mammary ductal carcinoma cells revealing its therapeutic potential. Oncotarget. 2017 May 30;8(22):35835-35847. PMID: 28415766; PMCID: PMC5482621.
  7. Bhat S, Sood R, Shukla S, Khandia R, Pateriya AK, Kumar N, Singh VK, Kalaiyarasu S, Kumar M, Bhatia S. A two dose immunization with an inactivated reassortant H5N2 virus protects chickens against lethal challenge with homologous 2.3.2.1 clade and heterologous 2.2 clade highly pathogenic avian influenza H5N1 viruses. Vet Microbiol. 2018 Apr;217:149-157. PMID: 29615248.
  8. Khangembam Victoria Chanu, Dimpal Thakuria, Richa Sood, Atul K. Pateriya, Sandeep Bhatia, Satish Kumar (2017). Identification of immunodominant epitopes in the HA2 subunit of H5N1 haemagglutinin by immunoassay using synthetic peptides as antigens. Chem. Biol. Lett. 2017, 4(1), 20-26. http://krishi.icar.gov.in/jspui/handle/123456789/5982.
  9. Bhatia S, Khandia R, Sood R, Bhat S, Siddiqui A, Jahagirdhar G, Mishra S, Mishra A, Pateriya AK, Kulkarni DD. Reverse genetics based rgH5N2 vaccine provides protection against high dose challenge of H5N1 avian influenza virus in chicken. Microb Pathog. 2016 Aug;97:172-7. PMID: 27296706.
  10. Jacob A, Sood R, Chanu KhV, Bhatia S, Khandia R, Pateriya AK, Nagarajan S, Dimri U, Kulkarni DD. Amantadine resistance among highly pathogenic avian influenza viruses (H5N1) isolated from India. Microb Pathog. 2016 Feb;91:35-40. PMID: 26639679.
  11. Mishra N, Rajukumar K, Pateriya A, Kumar M, Dubey P, Behera SP, Verma A, Bhardwaj P, Kulkarni DD, Vijaykrishna D, Reddy ND. Identification and molecular characterization of novel and divergent HoBi-like pestiviruses from naturally infected cattle in India. Vet Microbiol. 2014 Nov 7;174(1-2):239-46. PMID: 25301283.
  12. Sengupta D, Shaikh A, Bhatia S, Pateriya AK, Khandia R, Sood R, Prakash A, Pattnaik B, Pradhan HK. Development of single-chain Fv against the nucleoprotein of type A influenza virus and its use in ELISA. J Virol Methods. 2014 Nov;208:129-37. PMID: 25152529.
  13. Kamboj A, Pateriya AK, Mishra A, Ranaware P, Kulkarni DD, Raut AA. Novel molecular beacon probe-based real-time RT-PCR assay for diagnosis of Crimean-Congo hemorrhagic fever encountered in India. Biomed Res Int. 2014;2014:496219. PMID: 24877102; PMCID: PMC4022296.
  14. Sood R, Khandia R, Bhatia S, Hemadri D, Kumar M, Patil SS, Pateriya AK, Siddiqui A, Kumar MS, Venkatesha MD, Kulkarni DD. Detection and molecular characterization of naturally transmitted sheep associated malignant catarrhal fever in cattle in India. Trop Anim Health Prod. 2014 Aug;46(6):1037-43. PMID: 24817424.
  15. Gupta V, Mishra N, Pateriya A, Behera SP, Rajukumar K. Peripheral blood mononuclear cells from field cattle immune to bovine viral diarrhea virus (BVDV) are permissive in vitro to BVDV. Acta Virol. 2014;58(2):114-9. PMID: 24957715.
  16. Bhat S, Bhatia S, Sood R, Bhatnagar H, Pateriya A, Venkatesh G. Production and characterization of monoclonal antibodies against nucleoprotein of avian influenza virus. Monoclon Antib Immunodiagn Immunother. 2013 Dec;32(6):413-8. PMID: 24328746.
  17. Bhatia S, Kunal A, Khandia R, Siddiqui A, Pateriya AK, Sood R. Genetic and antigenic analysis of H5N1 viruses for selection of HA-donor virus for vaccine strains. Indian J Virol. 2013 Dec;24(3):357-64. doi: 10.1007/s13337-013-0151-2. PMCID: PMC3832698.
  18. Khandia R, Pattnaik B , Rajukumar K, Pateriya A K, Murugkar H V, Bhatia S, Prakash A and Pradhan H K (2013). Evaluation of a protective antigen gene based SYBR Green I real time PCR for detection of Bacillus anthracis in field samples. Indian Journal of Animal Sciences 83: 118–123. http://krishi.icar.gov.in/jspui/handle/123456789/5956.
  19. Sood R, Kumar M, Bhatia S, Pateriya AK, Khandia R, Siddiqui A, Kulkarni DD, Kumar S, Venkatesha MD. Ovine herpesvirus type 2 infection in captive bison in India. Vet Rec. 2012 Jun 23;170(25):654. PMID: 22730503.
  20. Ranaware PB, Kulkarni DD, Mishra A, Pateriya A, Gandhale PN, Singh B, Kumar VP, Tosh C, Raut AA. (2013) Detection of Highly Pathogenic Avian Influenza H5N1 Viral Load by Real-Time RT-PCR in Tissues of Experimentally Infected Chickens. Journal Veterinary Public Health, 11 (2).http://krishi.icar.gov.in/jspui/handle/123456789/5960.
  21. Ranaware PB, Kulkarni DD, Mishra A, Pateriya A, Gandhale PN, Kumar VP, Tosh C, Murugkar HV, Raut AA (2013) Genetic diversification of highly pathogenic avian influenza (HPAI) H5N1 virus in experimentally infected domestic ducks. Veterinary Research International. 1(2): 33-40. http://krishi.icar.gov.in/jspui/handle/123456789/5959.
  22. Vanamayya, P., Pattnaik, B., Pateriya, A., Desai, G., & Rajukumar, K. (2011). Genomic detection methods for detection of pseudorabies virus infection in Indian pigs. The Indian Journal of Animal Sciences, 79(12). Retrieved from http://epubs.icar.org.in/ejournal/index.php/IJAnS/article/view/2999
  23. Tosh C, Murugkar HV, Nagarajan S, Bhatia S, Pateriya AK, Behera P, Jain R, Kumar S, Khandia R, Vanamayya PR, Dubey SC, Ahlawat SP. Outbreak of avian influenza virus H5N1 in India. Vet Rec. 2007 Aug 25;161(8):279. PMID: 17720970.
  24. Santhosh SR, Parida MM, Dash PK, Pateriya A, Pattnaik B, Pradhan HK, Tripathi NK, Ambuj S, Gupta N, Saxena P, Lakshmana Rao PV. Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantitation of Japanese encephalitis virus. J Virol Methods. 2007 Jul;143(1):73-80. PMID: 17403544.
  25. Santhosh SR, Parida MM, Dash PK, Pateriya A, Pattnaik B, Pradhan HK, Tripathi NK, Ambuj S, Gupta N, Saxena P, Lakshmana Rao PV. Development and evaluation of SYBR Green I-based one-step real-time RT-PCR assay for detection and quantification of Chikungunya virus. J Clin Virol. 2007 Jul;39(3):188-93. PMID: 17553740.
  26. Pattnaik, B., Pateriya, A., Khandia, R., Tosh, C., Nagarajan, S., Gounalan, S., Murugkar,H.V., Shankar B, P., Shrivastava, N., Behera, P., Bhagat, S., Peiris J.SM., Pradhan, H. (2006). Phylogenetic analysis revealed genetic similarity of the H5N1 avian influenza viruses isolated from HPAI outbreaks in chickens in Maharashtra, India with those isolated from swan in Italy and Iran in 2006. Current Science, 91(1), 77-81. Retrieved January 10, 2021, from http://www.jstor.org/stable/24094179

Book Chapters/ Training Manuals

  1. RautA.A., Gandhale P.N., Senthil Kumar D., Kumar N., Pateriya A.K., Mishra A. and Kulkarni D.D., (2019).Crimean-Congo Haemorrhagic Fever Virus: Emerging and Transboundary Animal Viruses, Yashpal Singh Malik et al.(eds) Springer Nature, Singapore Pte Ltd. pp 978-981.

    2. Membership/Fellowship of Academic Societies

    1. Lifetime Member, Society for Biosafety, India.
    2. Lifetime Member, Association of Avian Health Professionals.

Research Projects Handled - 16

(International - 01, Contractual research project - 01, DBT/DST - 03, NABARD - 01, ICAR Scheme - 03, ICAR- Institutional - 06)

S.No.
Name Of the Project
 Funding Agency
1 Service project on "Surveillance of exotic and emerging animal diseases in Indian and imported livestock and poultry and their products" (2007- till date) (Co-PI) ICAR
2 Development of Lateral Flow Assay for detection of Avian Influenza infection in chicken (2018-2021) (PI) ICAR
3 Development of broadly protective H5 and H9 avian influenza vaccine (2019-2021) (Co-PI) ICAR
4 Development and validation of LFA POCT kits for SARS-COV-2 Antigen detection. (2020) (PI) BIRAC
5 Indo-US collaborative research project under Agricultural Knowledge Initiative (AKI) on Development and evaluation of protective efficacy of neuraminidase DIVA marker vaccines against Indian isolates of highly pathogenic H5N1 avian influenza viruses in chickens. (Co- PI). (2008-2010) (Co-PI) ICAR-Indo-US
6 Development of Yeast Expressed Recombinant Antigens for Diagnosis and Immunoprophylaxis of Avian influenza Virus (2007-2010) (Co-PI) ICAR
7 Development of an immuno-chromatographic test for the diagnosis of AIV infection in chicken (2010-2013 ) (Co-PI) NABARD
8 Identification of Neuraminidase inhibitor drug resistance in H5N1 viruses isolated from India. (2012-2015). (Co-PI) ICAR
9 Quest For A Universal Diagnostic PCR Array For Multiple Exotic And Emerging Viral Diseases (2012-2014). (Co-PI) ICAR
10 Contractual research Project on Prevalence of Bovine Viral Diarrhoea Virus (BVDV) types and subtypes in cattle in organized dairy farms in India (2012-14). (Co-PI) Pfizer Pct Ltd
11 Synthetic peptide based diagnostic for highly pathogenic avian influenza. (Co-PI) (2014-17) DBT
12 CRP on Vaccines and Diagnostics- Development of DNA strip test for sub-typing of avian influenza viruses. (2015-2018) (Co-PI) ICAR
13 CRP on Vaccines and Diagnostics- Development of recombinant nucleoprotein based ELISA for Avian Influenza antibody detection (2015-2020) (Co-PI) ICAR
14 DBT-NER-Advanced Animal Disease Diagnosis and Management Consortium (2014-2020) (Co-PI) DBT
15 Rapid visual diagnostic assay for influenza A viruses using gold nanoparticles and peptide nucleic acid (2016-2018) (Co-PI) ICAR
16 CRP on Vaccines and Diagnostics- Development of NS3 specific monoclonal antibody based BVDV diagnostic. (2018-2021) (Co-PI) ICAR

Equal Opportunity for Persons with Disabilities

In accordance with the provisions of the Right of Persons with Disabilities Act, 2016 and Rules, the institute strives to provide opportunities and facilities to persons with disabilities to participate, perform and excel in their work on an equal basis in everyday life.