Publications

1. M. Kumar, H. V. Murugkar, S. Nagarajan, C. Tosh, Sandeep Patil, K. H. Nagaraja, K Rajukumar, Senthilkumar, D & S. C. Dubey (2020). Experimental infection and pathology of two highly pathogenic avian influenza H5N1 viruses isolated from crow and chicken in house crows (Corvus splendens). ActaVirologica, 64(3):325-330. doi: 10.4149/av_2020_306.
2. Nayakwadi, S., Ramu, R., Sharma, A.K., Gupta, V.K., Rajukumar, K., Kumar, V., Shirahatti, P.S., Rashmi L. and Basalingappa, K.M. (2020) Toxicopathological studies on the effects of T-2 mycotoxin and their interaction in juvenile goats, PLOS ONE 15(3): e0229463. https://doi.org/10.1371/journal.pone.0229463.
3. Kombiah, S., Kumar, M., Murugkar, H. V., Nagarajan, S., Tosh, C., Kumar, D. S., Rajukumar, K., Gautam, S., Singh, R., Karikalan, M. and Sharma, A. K. (2020). Experimental pathology of two highly pathogenic H5N1 viruses isolated from crows in BALB/c mice. Microbial Pathogenesis, 141, 103984. https://doi.org/10.1016/j.micpath.2020.103984
4. Pandey M.K., Rajukumar K., Senthilkumar D., Kombiah S., Shrivas S. and Singh V.P. (2019) Age related changes in lymphocyte sub-populations in peripheral blood of healthy weaned piglets, Indian Journal of Veterinary Pathology, 43(1), 23-27. Doi: 10.5958/0973-970X.2019.00005.1.
5. Kalaiyarasu S, Mishra N, Rajukumar K, Behera SP, Jhade SK and Singh VP (2019) Development and evaluation of real-time RT-PCR using ear hair for specific detection of sheep persistently infected with border disease virus (BDV). Journal of Virological Methods, 269, 55-63. doi: 10.1016/j.jviromet.2019.04.003.
6. Senthilkumar D, Rajukumar K, Kumar M, Kalaiyarasu S, Shrivastava D, Katare M, Kulkarni DD, Singh VP. (2018) Porcine reproductive and respiratory syndrome virus induces concurrent elevation of High Mobility Group Box-1 protein and pro-inflammatory cytokines in experimentally infected piglets. Cytokine. pii: S1043-4666(18)30246-1. doi: 10.1016/j.cyto.2018.06.002.
7. Senthilkumar D, Rajukumar K, Sen A, Kumar M, Shrivastava D, Kalaiyarasu S, Gautam S, Singh F, Kulkarni DD, Singh VP. (2018) Pathogenic characterization of porcine reproductive and respiratory syndrome virus of Indian origin in experimentally infected piglets. Transbound Emerg Dis. doi: 10.1111/tbed.12893.
8. Mishra N, Behera SP, Kalaiyarasu S, Nema RK, Pandey P and Rajukumar K. (2018). Complete genome sequence analyses of an indian cattle strain of bovine viral diarrhoea virus 2 (BVDV-2). Indian Journal of Experimental Biology. 56. 322-326.
9. Dash SK, Kumar M, Kataria JM, Nagarajan S, Tosh C, Murugkar HV, Rajukumar K, Kalaiyarasu S, Senthil Kumar D, Kulkarni DD. (2018) Induction profiles of mRNA of toll like receptors and cytokines in chickens pre-exposed to low pathogenic avian influenza H9N2 virus followed by challenge with highly pathogenic avian influenza H5N1 virus. Microbial Pathogenesis. 117:200-205. doi: 10.1016/j.micpath.2018.02.041.
10. Vasudevan G, Vanamayya PR, Nagarajan S, Rajukumar K, Suba S, Venketash G, Tosh C, Sood R, Nissly RH and Kuchipudi SV (2017) Infectious dose-dependent accumulation of live highly pathogenic avian influenza H5N1 virus in chicken skeletal muscle - implications for public health, Zoonoses Public Health DOI: 10.1111/zph.12406.
11. Senthilkumar D, Rajukumar K., Jeena K, Kalaiyarasu S, Manoj Kumar, Shrivastava D, Bano HF, Diwakar Kulkarni D and Singh VP (2017) Pulmonary alveolar macrophages reveal higher basal cytokine mRNA expression than peripheral blood leucocytes in healthy piglets, Journal of Animal Research, 7: 629-634.
12. Singh V, Mishra N, Kalaiyarasu S, Khetan RK, Hemadri D, Singh RK, Rajukumar K., Chamuah J, Suresh KP, Patil SS, Singh VP (2017) First report on serological evidence of bovine viral diarrhea virus (BVDV) infection in farmed and free ranging mithuns (Bos frontalis), Trop Anim Health Prod., 49(6):1149-1156.
13. Khandia R, Pattnaik B, Rajukumar K., Pateriya A, Bhatia S, Murugkar H, Prakash A, Pradhan HK, Dhama K, Munjal A. and Joshi SK. (2017) Anti-proliferative role of recombinant lethal toxin of Bacillus anthracis on primary mammary ductal carcinoma cells revealing its therapeutic potential, Oncotarget. 8(22):35835-35847.
14. Patel SK, Rajukumar K, Kumar R, Reddy BVR, Singh R, Munuswamy P, Rana J (2016). Histopathological changes in grossly normal caprine mesenteric lymph nodes. Adv. Anim. Vet. Sci. 4(1): 53-56.
15. Karikalan M, Rajukumar K, Mishra N, Kumar M, Kalaiyarasu S, Rajesh K, Gavade V, Behera SP and Dubey SC (2016) Distribution pattern of bovine viral diarrhoea virus type 1 genome in lymphoid tissues of experimentally infected sheep, Veterinary Research Communications. DOI: 10.1007/s11259-016-9654-9.
16. Rajawat S, Kurchania R, Rajukumar K, Pitale S, Saha S and Qureshi MS (2016) Study of anti-cancer properties of green silver nanoparticles against MCF-7 breast cancer cell lines. Green Processing and Synthesis. DOI: 10.1515/gps-2015-0104.
17. Pavulraj S, Bera BC, Joshi A, Anand T, Virmani M, Vaid RK, Shanmugasundaram K, Gulati BR, Rajukumar K, Singh R, Misri J, Singh RK, Tripathi BN, Virmani N.(2015) Pathology of Equine Influenza virus (H3N8) in Murine Model. PLoS One. 10(11):e0143094. doi: 10.1371/journal.pone.0143094.
18. Anand T, Vaid RK, Bera BC, Singh J, Barua S, Virmani N, Rajukumar K, Yadav NK, Nagar D, Singh RK, Tripathi BN. (2016) Isolation of a lytic bacteriophage against virulent Aeromonas hydrophila from an organized equine farm. J Basic Microbiol. doi: 10.1002/jobm.201500318.
19. Mishra N, Rajukumar K, Vilcek S, Kalaiyarasu S, Behera SP, Dubey P, Nema RK, Gavade VB, Dubey SC, Kulkarni DD. (2016) Identification and molecular characterization of border disease virus (BDV) from sheep in India. Comparative Immunology, Microbiology and Infectious Diseases. 44, 1–7.
20. Athira CK, Murugkar HV, Kumar M, Nagarajan S, Tosh C, Bhatia, S Rajukumar K and Kumar, A (2015). Evaluation of elution methods for recovery of highly pathogenic avian influenza (H5N1) virus from infected duck feathers. Indian Journal of Animal Sciences 85 (12): 1303–1305. DOI- 10.10007/S13337-013-0151-2.
21. Rajukumar, K, Karikalan, M., Kumar, M., Mishra, N., Behera, S.P. and Kulkarni, D.D. (2015) Comparison of immune electron microscopy with direct negative staining for detection of Bovine Viral Diarrhoea Virus, Ind J Vet Pathol., 39:3, 235-238. DOI:10.5958/0973-970X.2015.00056.5.
22. Behera SP, Mishra N, Nema RK, Pandey PD, Kalaiyarasu S,Rajukumar, K.,Prakash A (2015) Expression of Bovine Viral Diarrhea Virus Envelope Glycoprotein E2 in Yeast Pichia pastoris and its Application to an ELISA for Detection of BVDV Neutralizing Antibodies in Cattle. J Immunoassay Immunochem. 36:639-54.
23. Suba S, Nagarajan S, Saxena VK, Kumar M, Vanamayya PR, Rajukumar K, Gowthaman V, Jain V, Singh DP, Dubey SC (2015) Pathology of a H5N1, highly pathogenic avian influenza virus, in two Indian native chicken breeds and a synthetic broiler line. Indian J Exp Biol. 53:202-7.
24. Dubey, P., Mishra, N., Rajukumar, K., Behera, S.P., Kalaiyarasu, S., Nema, R.K., Prakash, A. (2015) Development of a RT-PCR ELISA for simultaneous detection of BVDV-1, BVDV-2 and BDV in ruminants and its evaluation on clinical samples. Journal of Virological Methods. 213:50-6.
25. Kalaiyarasu S, Mishra N, Rajukumar K, Nema RK, Behera SP. (2015) Development and evaluation of a truncated recombinant NS3 antigen-based indirect ELISA for detection of pestivirus antibodies in sheep and goats. Journal of Immunoassay and Immunochemistry, 36(3):312-23. doi: 10.1080/15321819.2014.947433.
26. Mishra, N., Rajukumar, K., Pateriya, A., Kumar, M., Dubey, P., Behera, S.P., Verma, A., Bhardwaj, P., Kulkarni, D.D., Vijaykrishna, D. and Reddy, N.D. (2014) Identification and molecular characterization of novel and divergent HoBi-like pestiviruses from naturally infected cattle in India. Veterinary Microbiology. 174 (1-2):239-46.
27. Gupta, V., Mishra, N., Rajukumar, K., Behera, S. P., Dubey, P. (2014). Detection of bovine viral diarrhoea virus (BVDV) in peripheral blood mononuclear cells (PBMCs) of BVDV antibody positive Indian cattle following mitogen stimulation. Ind J Anim Sci. 84: 156-158.
28. Kamboj A, Raut AA, Senthilkumar D, Ranaware P, Rajukumar K, Mishra A, Kulkarni DD (2013) Amplification and Cloning of Full length ‘S’ Segment of Crimean-Congo Hemorrhagic Fever Virus from Hyalomma ticks infesting cattle in Gujarat, India. J. Vet. Pub. Health, 11 (2): 141-14.
29. Khandia R ,Pattnaik B , Rajukumar K, Pateriya A K, Murugkar H V, Bhatia S, Prakash A and Pradhan H K (Feb 2013). "Evaluation of a protective antigen gene based SYBR Green I real time PCR for detection of Bacillus anthracis in field samples”. Indian Journal of Animal Sciences 83 (2): 118–123.
30. Mishra N., Pitale, S.S., Rajukumar, K., Prakash, A., Behera, S.P., Nema, R.K. and Dubey SC, 2012, Genetic variety of bovine viral diarrhea virus 1 strains isolated from sheep and goats in India. Acta Virol. 56, 209-15.
31. Mishra, N., Rajukumar, K., Kalaiyarasu, S., Behera, S.P., Nema, R.K., Dubey, S.C. 2011. Small interfering RNAs targeting viral structural envelope protein genes and the 5’-UTR inhibit replication of bovine viral diarrhoea virus in MDBK cells. Acta Virologica. 55: 279-282.
32. Sarma, D.K., Mishra, N., Vilcek, S., Rajukumar, K., Behera, S.P., Nema, R.K., Dubey, P. and Dubey, S.C. 2011. Phylogenetic analysis of recent classical swine fever virus (CSFV) isolates from Assam, India. Comparative Immunology, Microbiology and Infectious Diseases. 34: 11-15.
33. Behera, S.P., Mishra, N., Vilcek, S., Rajukumar, K., Nema, R.K., Prakash, A., Kalaiyarasu, S., Dubey, S.C. 2011. Genetic and antigenic characterization of bovine viral diarrhoea virus type 2 isolated from cattle in India. Comparative immunology, microbiology and infectious diseases. 34:189-196.
34. Mishra, N., Rajukumar, K., Pitale, S.S., Prakash, A., Nema, R.K., Behera, S.P., Dubey, S.C. (2010). Evidence of a humoral immune response against the prokaryotic expressed N-terminal autoprotease (Npro) protein of bovine viral diarrhoea virus. Journal of Biosciences, 35: 79-86.
35. Mathapati, B.S., Mishra, N., Rajukumar, K., Nema, R.K., Behera, S.P., Dubey, S.C. (2010). Entry of bovine viral diarrhea virus into ovine cells occurs through clathrin-dependent endocytosis and low pH-dependent fusion. In Vitro cellular & developmental biology. Animal, 46:403-407.
36. Mishra, N., Mathapati, B.S., Rajukumar, K., Nema, R.K., Behera, S.P., Dubey, S.C. (2010). Molecular characterization of RNA and protein synthesis during a one-step growth curve of bovine viral diarrhoea virus in ovine (SFT-R) cells. Research in Veterinary science, 89: 130-2.
37. Mishra, N., Rajukumar, K., Tiwari, A., Nema, R.K., Behera, S.P., Satav, J.S. and Dubey, S.C. (2009) Prevalence of Bovine viral diarrhoea virus (BVDV) antibodies among sheep and goats in India. Tropical Animal Health and Production. 41: 1231-1239.
38. Vanamayya, P.R., Pattnaik, B., Pateriya, A. K., Desai, G.S., Thomas, R. and Rajukumar, K. (2009) Genomic detection methods for the detection of pseudorabies virus infection in Indian pigs. Indian Journal of Animal Sciences, 79, 1200-1214.
39. Mishra, N., Rajukumar, K., Vilcek, S., Tiwari, A., Satav, J.S. and Dubey, S.C. (2008) Molecular characterization of bovine viral diarrhea virus type 2 isolate originating from a native Indian sheep (Ovies aries), Veterinary Microbiology, 130, 88-98.
40. Nagarajan, S., Rajukumar, K., Tosh, C., Ramaswamy, V., Purohit, K., Saxena, G., Behera, P., Pattnaik, B., Pradhan, H.K. and Dubey S.C. (2008) Isolation and pathotyping of H9N2 avian influenza viruses in Indian poultry. Veterinary Microbiology, 133: 154-163.
41. Mishra, N., Vilcek, S., Rajukumar, K., Dubey, R., Tiwari, A., Galav, V. and Pradhan, H.K. (2008) Identification of bovine viral diarrhea virus type 1 in yaks (Bos poephagus grunniens) in the Himalayan region, Research in Veterinary Science, 84, 507-510.
42. Tosh, C., Nagarajan, S., Behera, P., Rajukumar, K., Purohit, K., Kamal, R.P., Murugkar, H.V., Gounalan, S., Pattnaik, B., Vanamayya, P.R., Pradhan, H.K., Dubey, S.C. (2008) Genetic analysis of H9N2 avian influenza viruses isolated from India, Archives of Virology, 153, 1433-1439.
43. Sarma, D.K., Mishra, N., Rajukumar, K., Sarma, S. and Singh, M.K. (2008). Isolation and characterization of classical swine fever virus from pigs in Assam, Indian Journal of Animal Sciences, 78, 37-37.
44. Mishra, N., Dubey, R., Rajukumar, K., Tosh, C., Tiwari, A., Pitale, S. S., Pradhan, H. K. (2007) Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus). Veterinary Microbiology, 124, 378-387.
45. Galav, V., Mishra, N., Dubey, R., Rajukumar, K., Pitale, S.S., A.B. Shrivastav and Pradhan, H.K. (2007) Pathogenicity of an Indian isolate of bovine viral diarrhea virus 1b in experimentally infected calves, Research in Veterinary Science, 83, 364-368.
46. Mishra, N., Dubey, R., Galav, V., Tosh, C., Rajukumar, K., Pitale, S.S. and Pradhan, H.K. (2007) Identification of bovine viral diarrhea virus type 1 in Indian buffaloes and their genetic relationship with cattle strains in 5’ UTR, Current Science, 93, 97-100.
47. Gounalan, S., Rajukumar, K., Bhatia, S., Sood, R., Nagarajan, S., Pattnaik B. and Pradhan, H.K. (2007) Enzyme linked immunosorbent assay and polymerase chain reaction based surveillance of rabbit haemorrhagic disease in Indian and imported rabbits. Indian Journal of Animal Sciences, 78, 263-265.
48. Kurade, N. P., Rajukumar, K. and Tripathi, B. N. (2007) Distribution of histologically detectable iron complexes in small intestine and mesenteric lymph node lesions of paratuberculosis in sheep and goats, Indian Journal of Veterinary Pathology,31, 36-39.
49. Sundaresan, N.R., Sukumar, K., Rajukumar, K., Nagarajan, K., Jaganathan, S. and Dorairajan, N. (2007) Antimicrobial susceptibility of bacterial isolates from sheep colostrums in Tamil Nadu state (India), Research Journal of Microbiology, 2, 198-200.
50. Rajukumar, K., Tripathi, B. N., Kurade, N. P. and Parihar, N. S. (2006) A dot enzyme immunoassay for large scale screening of sera for antibodies to Mycobacterium avium subsp. paratuberculosis in goats. Journal of Applied Animal Research, 29, 49-52.
51. Rajukumar, K., Sharma, A. K., Sundaresan, N. R., Sharma, B. and Dwivedi, P. (2006) In vitro studies to evaluate apoptosis induction by very low doses of ochratoxin A in rabbit splenocytes and peripheral blood mononuclear cells. Indian Journal of Veterinary Pathology, 30, 16-20.
52. Sukumar, K., Sundaresan, N.R., Rajukumar, K., Nagarajan, K., Sukumar, K. and Dorairajan, N. (2006) Identification and antibiotic sensitivity pattern of pathogenic bacteria isolated from dead-in-shell embryos. Indian Journal of Poultry Science, 41, 105-107.
53. Rajukumar, K. and Tripathi, B. N. (2005) Histopathological spectrum and its relationship with humoral immune responses in naturally occurring caprine paratuberculosis. Indian Journal of Veterinary Pathology, 29, 71-77.
54. Kurade, N.P., Tripathi, B.N., Rajukumar, K., and Parihar, N.S. (2004) Sequential development of histological lesions and their relationship with bacterial isolation, faecal shedding and immune responses during progressive stages of experimental infection of lambs with Mycobacterium avium subsp. Paratuberculosis, Veterinary Pathology, 41, 378-387.
55. Rajukumar, K., Tripathi, B.N., Kurade, N.P. and Parihar, N.S. (2000) An enzyme-linked immunosorbent assay using immunoaffinity-purified antigen in the diagnosis of caprine paratuberculosis and its comparison with conventional ELISAs, Veterinary Research Communications, 25, 539-553.
56. Tripathi, B.N., Rajukumar, K., Kurade, N.P. and Parihar, N.S. (2000) Prevalence of Mycobacterium avium paratuberculosis infection in goats and sheep based on the faecal culture, Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases, 21:1, 72-73.
57. Tripathi, B.N., Rajukumar, K., Kurade, N.P. and Parihar, N.S. (2000) Prevalence of Mycobacterium avium paratuberculosis infection in goats and sheep based on the faecal culture, Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases, 21:1, 72-73.

Technical Bulletin

1. N. Mishra, K. Rajukumar and S. C. Dubey (2007). Bovine Viral Diarrhoea (BVD) and its diagnosis.

Review Article

1. Mishra, N., Rajukumar, K., Kalaiyarasu, S., Dubey, S. C., 2011. Pestivirus infection, an emerging threat to ruminants in India: A review. Ind. J. Anim. Sci. 81:545-551.

Training/Laboratory Manual

1. Training manual. 2014. Eds. Raut, A. A., Nagarajan, S., Venkatesh, G., Mishra, A., Sood, R., Kumar, M. and Rajukumar, K. International Training cum Workshop on "Conventional and Molecular techniques for diagnosis of trans-boundary animal diseases" for the ASEAN countries organized at HSADL, IVRI, Bhopal from February 18 to March 7, 2014.
2. Training manual on laboratory protocols. 2013. Eds. Rajukumar, K., Nagarajan, S., Pateriya, A. K. and Murugkar, H. V. SAARC regional training programme on "Molecular techniques in diagnosis of diseases of farm animals and poultry" for SAARC countries organized at HSADL, IVRI, Bhopal from April 22 to May 1, 2013.
3. Mishra, N., Rajukumar, K., Kalaiyarasu, S., Dubey, S. C., 2011. Laboratory Manual for diagnosis of bovine viral diarrhoea (BVD), published by HSADL, IVRI, Bhopal p.1-30.

General Technical Articles

1. Rajukumar K, Manoj Kumar, Senthil Kumar D (2014) Immunoperoxidase tests for detection of Porcine Reproductive and Respiratory Syndrome and Avian Influenza viruses, In: training manual : International Training-cum-Workshop for ASEAN region on "Conventional and Molecular Techniques for Diagnosis of Transboundary Animal Diseases", HSADL, IVRI, Bhopal, India, 18th February - 7th March 2014.
2. Manoj Kumar, Senthil Kumar D. Rajukumar K (2014) Transmission electron microscopy for viral disease diagnosis, In: training manual : International Training-cum-Workshop for ASEAN region on "Conventional and Molecular Techniques for Diagnosis of Transboundary Animal Diseases", HSADL, IVRI, Bhopal, India, 18th February - 7th March 2014.
3. Rajukumar, K. (2013) Transmission electron microscopy: Basics and applications in diagnosis of viral diseases, chapter in training manual on "Molecular techniques in diagnosis of diseases of farm animals and poultry", SAARC Regional Training Programme, HSADL, IVRI, Bhopal, April 22 to May 1, 2013. pp 38-44.
4. Rajukumar, K. and Karikalan, M. (2013) In situ antigen and genome detection methods in disease diagnosis, chapter in training manual on "Molecular techniques in diagnosis of diseases of farm animals and poultry", SAARC Regional Training Programme, HSADL, IVRI, Bhopal, April 22 to May 1, 2013. pp 80-91.
5. Rajukumar, K. (2012) Transmission Electron Microscopy: Basics and application in the study of nanoparticles, chapter in manual on "Application of nanotechnology in soil science and plant nutrition research", Indian Institute of Soil Science, Bhopal.
6. Mishra, N. and Rajukumar, K. (2008). Biotechnology in animal disease diagnosis in post genomic era. Book chapter in Proceedings of MEDBIO-2008 & Third Industry – Academia Meet on "Status of Medical Biotechnology research in the State of Madhya Pradesh: Past accomplishments, Current Trends and Challenges Ahead" held at BMHRC, Bhopal, M.P., Eds. P. K. Mishra and K. K. Maudar, Feb 14-16, pp. 12-18.
7. Rajukumar, K. (2002). Porcine reproductive and respiratory syndrome: an overview, National seminar on exotic animal diseases organized at HSADL, Bhopal, April 1, pp 60-64.

Ongoing Project

1. Deployment of nucleic acid and ELISA based diagnostic tests to determine incidence of porcine reproductive and respiratory syndrome (PRRS) in the North East and evaluation of the prospects of a potential candidate vaccine against the disease (DBT funded) (Principal Investigator).
2. Development and evaluation of referral group specific and subtype specific diagnostic reagents for avian influenza (Co-Investigator).
3. Diagnosis and surveillance of BVD/BD among ruminants in India (Institute service project on Surveillance of exotic and emerging animal diseases in Indian and imported livestock & poultry and their products) (Co-Investigator).
4. Surveillance of Porcine reproductive and respiratory syndrome virus infection. (Institute service project on Surveillance of exotic and emerging animal diseases in Indian and imported livestock & poultry and their products) (Co-Investigator).

Completed Project

1. Alterations in immunocompetent cells and cytokines in the pathogenesis of BVDV infection in sheep (As PI) (DST funded).
2. Development of a one step Taq man realtime RT-PCR for detection and genetic typing of ruminant pestiviruses (as Co-PI).
3. Pathobiology of acute and persistent infection of bovine viral diarrhea in small ruminants.(As PI).
4. Sero-epidemiology and molecular diagnosis of type A influenza virus in poultry (as Co-PI).
5. Development of enzyme immunoassay and nucleic acid based diagnostic tests for the detection of porcine reproductive and respiratory syndrome in pigs. (As PI).