Dr. Niranjan Mishra

Principal Scientist

M.V.Sc., Ph.D., Veterinary Virology

Email : niranjan[dot]mishra1[at]icar[dot]gov[dot]in

Achievements

  1. First isolation and identification of BVDV-1 in cattle in India.
  2. First isolation and identification of BVDV-1 in yaks.
  3. First isolation and identification of BVDV-2b in sheep.
  4. First isolation and identification of BVDV-2 in Indian goats.
  5. First identification of BVDV-1 in Indian buffaloes.
  6. First determination that border disease in sheep and goats occurs in India due to BVDV-1 and BVDV.
  7. First determination of entry mechanism of BVDV-1 and BVDV-2 in ovine cells.
  8. First report of glomerulonephritis in calves due to acute BVDV infection.
  9. First determination of molecular epidemiology of BVDV in India.
  10. Generation of NS3 recombinant antigen from Indian BVDV isolate for seroprevalence of BVD.

Awards

  1. Hari Om Ashram Trust Award of Indian Council of Agricultural Research, Ministry of Agriculture, Govt. of India for the biennium 2005-2006 in Animal Science for outstanding contribution in BVDV research




International Journals

  1. Karikalan M, Rajukumar K, Mishra N, Kumar M, Kalaiyarasu S, Rajesh K, Gavade V, Behera SP and Dubey SC (2016) Distribution pattern of bovine viral diarrhoea virus type 1 genome in lymphoid tissues of experimentally infected sheep, Veterinary Research Communications. DOI: 10.1007/s11259-016-9654-9.
  2. Rajukumar, K., Karikalan, M., Kumar, M., Mishra, N., Behera, S.P. and Kulkarni, D.D. (2015) Comparison of immune electron microscopy with direct negative staining for detection of Bovine Viral Diarrhoea Virus, Ind J Vet Pathol., 39:3, 235-238. DOI:10.5958/0973-970X.2015.00056.5.
  3. Behera SP, Mishra N, Nema RK, Pandey PD, Kalaiyarasu S, Rajukumar K, Prakash A (2015) Expression of Bovine Viral Diarrhea Virus Envelope Glycoprotein E2 in Yeast Pichia pastoris and its Application to an ELISA for Detection of BVDV Neutralizing Antibodies in Cattle. J Immunoassay Immunochem. 36:639-54.
  4. Dubey, P., Mishra, N., Rajukumar, K., Behera, S.P., Kalaiyarasu, S., Nema, R.K., Prakash, A (2015) Development of a RT-PCR ELISA for simultaneous detection of BVDV-1, BVDV-2 and BDV in ruminants and its evaluation on clinical samples. Journal of Virological Methods. 213:50-6.
  5. Kalaiyarasu S, Mishra N, Rajukumar K, Nema RK, Behera SP. (2015) Development and evaluation of a truncated recombinant NS3 antigen-based indirect ELISA for detection of pestivirus antibodies in sheep and goats. Journal of Immunoassay and Immunochemistry, 36(3):312-23. doi: 10.1080/15321819.2014.947433.
  6. Mishra N, Rajukumar K, Pateriya A, Kumar M, Dubey P, Behera SP, Verma A, Bhardwaj P, Kulkarni DD, Vijaykrishna D, Reddy ND (2014) Identification and molecular characterization of novel and divergent HoBi-like pestiviruses from naturally infected cattle in India. Vet Microbiol. 174:239-46.
  7. Mishra, N., Kalaiyarasu, S., Nagarajan, S., Subba Rao, M. V., George, A., Sridevi, R., Behera, S.P., Dubey, S.C., McCracken, T., Newman, S.H. 2012. Serological evidence of West Nile virus infection in wild migratory and resident water birds in Eastern and Northern India. Comparative immunology, microbiology and infectious diseases. 35: 591-598.
  8. Mishra, N., Pitale, S. S., Rajukumar, K., Prakash, A., Behera, S. P., Nema, R. K. and Dubey, S.C. 2012. Genetic variety of bovine viral diarrhoea virus 1 strains isolated from sheep and goats in India. Acta Virologica. 56: 209-215.
  9. Mishra, N., Rajukumar, K., Kalaiyarasu, S., Behera, S.P., Nema, R.K., Dubey, S.C. 2011. Small interfering RNAs targeting viral structural envelope protein genes and the 5’-UTR inhibit replication of bovine viral diarrhoea virus in MDBK cells. Acta Virologica. 55: 279-282.
  10. Sarma, D.K., Mishra, N., Vilcek, S., Rajukumar, K., Behera, S.P., Nema, R.K., Dubey, P. and Dubey, S.C. 2011. Phylogenetic analysis of recent classical swine fever virus (CSFV) isolates from Assam, India. Comparative Immunology, Microbiology and Infectious Diseases. 34: 11-15.
  11. Behera, S.P., Mishra, N., Vilcek, S., Rajukumar, K., Nema, R.K., Prakash, A., Kalaiyarasu, S., Dubey, S.C. 2011. Genetic and antigenic characterization of bovine viral diarrhoea virus type 2 isolated from cattle in India. Comparative immunology, microbiology and infectious diseases. 34:189-196.
  12. Mishra, N., Rajukumar, K., Pitale, S. S., Prakash, A., Nema, R. K., Behera, S. P., Dubey, S. C. 2010. Evidence of a humoral immune response against the prokaryotic expressed N-terminal autoprotease (Npro) protein of bovine viral diarrhoea virus. Journal of Biosciences. 35 (1): 79-86.
  13. Mishra, N., Mathapati, B.S., Rajukumar, K., Nema, R.K., Behera, S.P., Dubey, S.C. 2010. Molecular characterization of RNA and protein synthesis during a one-step growth curve of bovine viral diarrhoea virus in ovine (SFT-R) cells. Research in Veterinary Science. 89: 130-132.
  14. Mathapati, B.S., Mishra, N., Rajukumar, K., Nema, R.K., Behera, S.P., Dubey, S.C. (2010).. Entry of bovine viral diarrhoea virus into ovine cells occurs through clathrin-dependent endocytosis and low pH-dependent fusion. In Vitro Cell Development Biology Animal. 46: 403-407.
  15. Mishra, N., Rajukumar, K., Tiwari, A., Nema, R.K., Behera, S.P., Satav, J.S. and Dubey, S.C. 2009. Prevalence of Bovine viral diarrhoea virus antibodies among sheep and goats in India. Tropical Animal health and Production. 41: 1231-1239.
  16. Mishra, N., Rajukumar, K. Vilcek, S., Tiwari, A., Satav, J. S., Dubey, S. C. 2008. Molecular characterization of bovine viral diarrhea virus type 2 isolate originating from a native Indian sheep (Ovies aries). Veterinary Microbiology. 130: 88-98.
  17. Mishra, N., Vilcek, S., Rajukumar, K. R., Dubey, R., Tiwari, A., Galav, V., Pradhan, H. K., 2008. Identification of bovine viral diarrhea virus type 1 in yaks (Bos poepaghus grunniens) in Himalayan region. Research in Veterinary Science. 84(3): 507-510.
  18. Bhatia, S., Sood, R., Mishra, N., Pattnaik, B., Pradhan, H. K., 2008. Development and evaluation of a MAb based competitive-ELISA using helicase domain of NS3 protein for sero-diagnosis of bovine viral diarrhea in cattle and buffaloes. Research in Veterinary Science. 85: 39-45.
  19. Mishra, N., Pitale, S.S. and Pradhan, H.K. 2008. Genetic analysis and expression of NS3 gene of bovine viral diarrhea virus type 1 from India for detection of antibodies in cattle. Journal of Applied Animal Research. 33: 99-103.
  20. Mishra, N., Dubey, R., Rajukumar, K., Tosh, C., Tiwari, A., Pitale, S. S., Pradhan, H. K. 2007. Genetic and antigenic characterization of bovine viral diarrhea virus type 2 isolated from Indian goats (Capra hircus). Veterinary Microbiology. 124: 340-347.
  21. Galav, V., Mishra, N., Dubey, R., Rajukumar, K., Pitale S.S., Shrivastav, A.B., Pradhan, H.K., 2007. Pathogenicity of an Indian isolate of bovine viral diarrhea virus 1b in experimentally infected calves. Research in Veterinary Science. 83(3): 364-368.
  22. Mishra, N., Vilcek S., Jain, P., Pitale, S. S. and Pradhan, H.K. 2006. Genetic analysis of Indian Bovine Viral Diarrhoea Virus Isolates in Npro and entire structural region genes. Acta Virologica. 50: 39-44.
  23. Mishra, N., Pattnaik, B., Vilcek, S., Patil, S.S., Jain, P., Swamy, N., Bhatia, S. and Pradhan, H.K. 2004. Genetic typing of bovine viral diarrhoea virus isolates from India. Veterinary Microbiology. 104 (3-4): 207-12.

National Journals

  1. Gupta, V., Mishra, N., Rajukumar, K., Behera, S. P., Dubey, P. (2014). Detection of bovine viral diarrhoea virus (BVDV) in peripheral blood mononuclear cells (PBMCs) of BVDV antibody positive Indian cattle following mitogen stimulation. Ind J Anim Sci. 84: 156-158.
  2. Sarma, D.K., Mishra, N., Rajukumar, K., Sarma, S. and Singh, N.K. 2008. Isolation and characterization of classical swine fever virus from pigs in Assam. Indian J. Anim. Sc. 78(1): 37-39.
  3. Mishra, N., Dubey, R., Galav, V., Tosh, C., Rajukumar, K., Pitale, S.S., Pradhan, H.K. 2007. Identification of bovine viral diarrhea virus 1 in Indian buffaloes and their genetic relationship with cattle strains in 5’ UTR. Current Science. 93: 97-100.
  4. Selvaraj, J., Murali Manohar B., Balachandran, C., Mishra, N., Pradhan, H.K. 2007. Seroprevalence of bovine viral diarrhoea in buffaloes at Chennai. Ind. J. Vet. Pathol. 31: 180.
  5. Mishra, N., Pitale, S. S., Jain, P. and Pradhan, H.K. 2005. Multiplex PCR in diagnosis and characterization of Bovine Viral Diarrhoea Virus isolates from India. Current Science. 88(1): 162-166.
  6. Pawaiya, R.V.S., Mishra, N., Bhagwan, P.S.K. and Dubey, S.C. 2004. Pathology and distribution of antigen in goats naturally infected with peste des petits ruminants virus. Ind.J.Anim.Sci. 74(1): 35-40.
  7. Patil, S.S., Pattnaik, B., Mishra, N., Bhanumathi, N., Dubey, R. and Pradhan, H.K.2003. Detection of proviral genomic sequence of Bovine immunodeficiency virus in Indian cattle. Current Science. 84(4): 563-566.
  8. Mishra, N., Mishra, S., Pawaiya, R.V.S. and Bhagwan, P.S.K. (2000). Isolation and characterization of Pasteurella haemolytica from a field outbreak in sheep of Rajasthan. Ind. J. Anim. Sci. 70(5) : 443-445.
  9. Das, S.C.; Mishra, N.; Mishra, S. and Bhagwan, P.S.K. (2000). An outbreak of enterotoxaemia in broiler rabbits in an organised farm. Ind. J. Comp. Microbiol. Immunol. Infect. Dis. 21(1): 155-56.
  10. Das, S.C., Mishra, S., Mishra, N. and Bhagwan, P.S.K. (1999). Comparison of capsular antigens of P. multocida strains. Ind. J. Anim. Sci. 69 (11) : 872-874.
  11. Mishra, S., Das, S.C., Mishra, N. and Bhagwan, P.S.K. (1999). Development of indirect-ELISA for study of immune response to P. multocida strains in sheep. Ind. J. Comp. Microbiol. Immunol. Infect. Dis. 20(1) : 70-71.
  12. Pawaiya, R.V.S., Mishra, N. and Bhagwan,P.S.K. (1999). Pathology of Bacillus piliformis infection in rabbits. Ind. J. Anim. Sci. 69 (11) : 924-927.
  13. Sen, A.R., Mishra, N. and Bhagwan, P.S.K. (1999). Quality changes during refrigerated storage of ground mutton treated with sodium acetate. Processed Food Ind. 2 (12): 31-48.
  14. Mishra, N.; Mishra, S. and Bhagwan, P.S.K.(1998). Reliability of faecal examination in diagnosis of Johne's disease in sheep. Ind. J. of Small Ruminants 4(1) : 37-39.
  15. Mishra, N., Das, S.C., Mishra, S. and Bhagwan, P.S.K. (1998). Use of indirect and dot-ELISA in detecting prevalence of bluetongue virus antibodies in sheep. Ind. J. Virol. 14 : 151-53.
  16. Pawaiya, R.V.S., Mishra, N., Singh, D., Swarnkar, C.P. and Bhagwan, P.S.K.(1998). Causes of rabbit mortality- a retrospective study. Ind. J. Vety. Pathol. 22 (2): 115-119.
  17. Das, S.C., Mishra, S., Mishra, N. and Bhagwan, P.S.K. (1997). Comparison of competitive ELISA, indirect-ELISA and Agar gel precipitation test for detection of bluetongue virus antibodies in sheep. Ind. J. Anim. Sci. 67(12): 1033-35.
  18. Mishra, N.; Mishra, S. and Bhagwan, P.S.K.(1997). Epidemiological observation of an outbreak of FMD in an organised goat farm. Ind. J. Comp. Microbiol. Immunol. Infect. Dis. 18(2):189.
  19. Mishra, S.; Mishra, N. and Bhagwan, P.S.K. (1997). Prevalence of E. coli serotypes in rabbits. Ind. J. Comp. Microbiol. Immunol. Infect. Dis, 18(2) : 196-97.
  20. Mishra, N.; Rai,D.V. and Pattnaik,B. (1995). Strain differentiation of Foot & Mouth Disease virus type Asia1. Indian J. Anim. Sci. 65(4): 368-374.
  21. Mishra, N.; Rai,D.V. and Pattnaik,B. (1995). Comparison of Foot & Mouth Disease virus Asia1 field isolates & vaccine virus by plaque assay and plaque reduction neutralization test. Indian J. Anim. Sci. 65(4): 375-379.

Review Article

  1. Mishra, N. 2014. Genetic and antigenic diversity of bovine viral diarrhoea viruses: Implications for diagnosis and management. Full invited paper, Veterinary pathology congress compendium 2013, p. 85-88. Bhubaneswar, 21-23 Nov.
  2. Mishra, N., Rajukumar, K., Kalaiyarasu, S., Dubey, S. C., 2011. Pestivirus infection, an emerging threat to ruminants in India: A review. Ind. J. Anim. Sci. 81:545-551.

Training/Laboratory Manual

  1. Mishra N. 2014. Chapters on virus isolation and identification of pestiviruses and Real-time RT-PCR for pestivirus diagnosis in Training manual for ASEAN countries on conventional and molecular techniques for diagnosis of transboundary animal diseases, HSADL, Bhopal, Feb. 2014.
  2. Mishra, N., Rajukumar, K., Kalaiyarasu, S., Dubey, S. C., 2011. Laboratory Manual for diagnosis of bovine viral diarrhoea (BVD), published by HSADL, IVRI, Bhopal p.1-30.

Training/ summer school conducted

  1. ICAR short course (10 days) on Molecular approaches for laboratory diagnosis of BVDV, 7-16th September, 2011, held at HSADL, IVRI, Bhopal. (Course Director: N. Mishra; Participants: 16).

GenBank submissions: 62

Technical articles : 5

Papers presented in International conferences: 16

Papers presented in National conferences: 14

Ongoing Project

  1. Diagnosis and surveillance of BVD/BD among ruminants in India (Institute service project on Surveillance of exotic and emerging animal diseases in Indian and imported livestock & poultry and their products).
  2. Diagnostic preparedness and surveillance for West Nile virus infection in migratory birds (Institute service project).

Completed Project

  1. Prevalence of bovine viral diarrhoea virus (BVDV) types and subtypes in cattle in organized dairy farms in India (Funded by M/s Pfizer India).
  2. Development of a one step Taq man realtime RT-PCR for detection and genetic typing of ruminant pestiviruses.
  3. DST funded project titled ‘Alterations in immunocompetent cells and cytokines in the pathogenesis of BVDV infection in sheep’.

Books

  • Dubey, S.C., Mishra, N., Shinde, A.K. 2012. Diseases of goat and sheep. Pp. 282. Publisher: DKMA, ICAR, KAB-1, New Delhi.

    • Book Chapters

    1. Mishra, N. 2014. West Nile Virus Infection. Chapter 5, in Zoonoses: Viral, Rickettsial and Prion diseases (eds. S. R. Garg). ISBN: 9789351301639. Daya Publishing House, New Delhi, P. 68-87.
    2. Mishra, N. 2012. Genetic and antigenic diversity of ruminant pestiviruses: implications for diagnosis and control. In Microorganisms in sustainable agriculture and biotechnology (Satyanarayana, T., Johri, B. N., Prakash, A. eds), Springer, New York, pp. 153-172.
    3. Mishra, N., Rajukumar, K. 2008. Biotechnology in animal disease diagnosis in post genomic era. Book chapter (Mishra, P.K. and Maudar, K.K.: Eds), BMHRC, Bhopal, M.P. pp. 12-18.

    Equal Opportunity for Persons with Disabilities

    In accordance with the provisions of the Right of Persons with Disabilities Act, 2016 and Rules, the institute strives to provide opportunities and facilities to persons with disabilities to participate, perform and excel in their work on an equal basis in everyday life.