Dr. Sandeep Bhatia

Principal Scientist

M.V.Sc., Ph.D., Veterinary Microbiology

Email : sandeepbhatia[at]hsadl[dot]nic[dot]in

Achievements

  1. ICAR National Fellow recognition.
  2. Diagnostic test for Bovine Immunodeficiency Virus, monoclonal antibody based competitive-inhibition ELISA, was developed under the Project entitled “Development of monoclonal antibodies and recombinant antibodies against BIV and their characterization”.
  3. Monoclonal antibody based competitive-inhibition ELISA test for diagnosis of Bovine Viral Diarrhoea Virus under the Project entitled “Molecular epidemiology and Immunobiology of Bovine viral diarrhoea virus (BVDV) infection in cattle and buffalo in India” (Funded by DBT) was developed.
  4. Development and characterization of monoclonal antibodies against NP protein of avian influenza.
  5. Development and characterization of recombinant antibodies against NP protein of avian influenza.
  6. Development of a killed vaccine against avian influenza.
  7. Development and characterization of monoclonal antibodies against NS1 protein of avian influenza.











National

  1. Athira CK, Murugkar HV, Kumar M, Nagarajan S, Tosh C, Bhatia, S Rajukumar K and Kumar, A (2015). Evaluation of elution methods for recovery of highly pathogenic avian influenza (H5N1) virus from infected duck feathers. Indian Journal of Animal Sciences 85 (12): 1303–1305. DOI- 10.10007/S13337-013-0151-2.
  2. Bhatia, S., Kunal, A., Khandia, R., Siddiqui, A., Pateriya, A. K., Sood, R. (2013). Genetic and antigenic analysis of H5N1 viruses for selection of HA donor virus for vaccine strain. Indian Journal of Virology. DOI- 10.10007/S13337-013-0151-2.
  3. Khandia R ,Pattnaik B , Rajukumar K, Pateriya A K, Murugkar H V, Bhatia S, Prakash A and Pradhan H K (Feb 2013). "Evaluation of a protective antigen gene based SYBR Green I real time PCR for detection of Bacillus anthracis in field samples”. Indian Journal of Animal Sciences 83 (2): 118–123.
  4. Sood, R., Hemadri, D., Bhatia, S. (2013). Sheep associated malignant catarrhal fever: an emerging disease of bovids in India. Indian Journal of Virology. DOI- 10.1007/s13337-013-0163-y.
  5. Bhatia, S., Patil, S.S., Sood, R. (2013). Bovine immunodeficiency virus: a lentiviral infection. Indian Journal of Virology. DOI-10.1007/s13337-013-0165-9.
  6. Sood, R., Swarup, D., Bhatia, S., Kulkarni, D.D., Dey, S., Saini, M., and Dubey, S. C. (2012). Antiviral activity of crude extracts of Eugenia jambolana Lam. against Highly pathogenic avian influenza (H5N1) virus. Indian J. of Exp. Biology, 50:179-186.
  7. Dubey, S.C., Nagarajan, S., Tosh, C., Bhatia, S. and Krishna, L., (2009). Avian influenza: a long-known disease and its current threat. Ind. J. Anim. Sci. 79 (2): 113-140 (review).
  8. S. Gounalan, K. Rajukumar, S. Bhatia, R. Sood, S. Nagarajan, B. Pattnaik and H. K. Pradhan (2008). Enzyme linked immunosorbent assay and polymerase chain reaction based surveillance of rabbit haemorrhagic disease in Indian and imported rabbits. Indian Journal of Animal Science 78 (3): 263-265.
  9. Bhatia, S., Patil, S. S., Sood, R., Dubey, R., Bhatia, A. K., Pattnaik, B. and Pradhan, H. K. (2008). Prokaryotic expression of a 750-bp capsid region of Bovine Immunodeficiency Virus gag gene and development of a recombinant capsid (p26) protein based immunoassay for seroprevalence studies. Indian journal of Biotechnology 7 (1) 50-55.
  10. Sood, R., Bhatia, S., Gounalan, S., Patil, S.S. and Pattnaik, B. (2007). Sero-prevalence of Bovine Viral Diarrhoea Virus in India: A survey from 1999-2004. Indian Journal Animal Sciences, 77, pp. 227-229.
  11. Sood, R., Bajpai, M. and Bhatia, S. (2007). Comparative efficacy of some acaricides in sarcoptic mange in Rabbits. Journal of Parasitology.
  12. Bhatia, S. and Bhatia, A.K. (2006). Recombinant phage display antibody technology - an alternative to hybridoma for antibody production. Journal of Immunology and Immunopathology, 8 (2).
  13. Bhatia S., Bhatia A. K., Sood R., Pattnaik B. and Pradhan H. K. (2006). Serological evidence of bovine immunodeficiency virus infection in cattle and buffalo through use of recombinant capsid (P26) protein based immunoassay. Journal of Immunology and Immunopathology, 8 (2), 128-129.
  14. Patil, S. S., Pattnaik, B., Desai, G. S., Banumathi, N., Bhatia, S., Sood, R., Vanamayya, P. R. and Pradhan, H. K. (2006). Biotinylated 5’ UTR probe for detection of bovine viral diarrhea virus (BVDV) sequences in persistently infected cattle. Indian J. Comp. Microbiol. Immunol. Infect. Dis. 27 (2): 59-61.
  15. Bhatia, S., and Das, S.K. (1999). Gamma interferon bioassay for detection of bovine tuberculosis in cattle: kinetics of production and dose response in whole blood culture. Ind. J. Exp. Biol., 37 (10): 1039-1041.

International

  1. Kumar N, Bera BC, Greenbaum BD, Bhatia S, Sood R, Selvaraj P, Anand T, Tripathi BN, Virmani N. (2016). Revelation of Influencing Factors in Overall Codon Usage Bias of Equine Influenza Viruses. PLoS One. 2016 Apr 27;11(4).e0154376.
  2. Khandia, R., Rajukumar, K., Pateriya, A., Bhatia, S., Murugkar, H., Pradhan, H.K., Prakash, A. and Pattnaik, B. (2014) In vitro evaluation of effect of B. anthracis lethal toxin on primary mammary tumor cells. American Journal of Infectious Diseases 10 (3): 132-137.
  3. Tosh C, Nagarajan S, Murugkar HV, Bhatia S, Kulkarni DD (2014). Evolution and Spread of Avian Influenza H5N1 Viruses. Advances in Animal and Veterinary Sciences 2 (4S): 33 – 41. (Review article).
  4. Sood R, Khandia R, Bhatia S, Hemadri D, Kumar M, Patil S. S, Pateriya A. K, Siddiqui A, Kumar M. S, Venkatesha M. D. and Kulkarni D. D.(2014) Detection and molecular characterization of naturally transmitted sheep associated malignant catarrhal fever in cattle in India. Tropical Animal Health and production. DOI 10.1007/s11250-014-0611-8.
  5. Bhat S, Bhatia S, Sood R, Bhatnagar H, Pateriya A, Venkatesh G. (2013). Production and characterization of monoclonal antibodies against nucleoprotein of avian influenza virus. Monoclonal Antibodies Immunodiagnosis Immunotherapy. 32(6):413-8.
  6. Sood, R., Kumar, M., Bhatia, S., Pateriya, A. K., Khandia, R., Siddiqui, A., Kulkarni, D. D., Kumar, S. and Venkatesha, M. D. (2012). Ovine herpesvirus type 2 infection in captive bison in India. Vet. Rec., 170: 654.
  7. Bhatia S., Gangil, R., Gupta, D. S., Sood, R., Pradhan, H.K. and Dubey, S. C. (2010). Single chain fragment variable antibody against the capsid protein of bovine immunodeficiency virus and its use in ELISA. J. Virol. Methods 167 (2010), pp. 68-73.
  8. Rai, M., Bhatia, S., Malik, Y.P.S., Dubey, S.C. (2010). Production and characterization of monoclonal antibodies against NS1 protein of H5N1 avian influenza virus. Hybridoma, 29 (2): 183-186.
  9. Gowthaman, V., Vanamayya, P.R., Nagarajan, S., Suba, S., Bhatia, S., Jain, R., Behera, P., Tosh, C., Murugkar, H.V., and Dubey, S.C., (2009) Influence of Dose of Inocula on Outcome of Clinical Disease in High-Pathogenicity Avian Influenza (H5N1) Infections- An Experimental Study. Avian Diseases, 54: 576–580.
  10. Bhatia S., Sood R., Bhatia, A. K., Pattnaik, B. and Pradhan H. K. (2008). Development of a capsid based competitive inhibition enzyme linked immunosorbent assay for detection of bovine immunodeficiency virus antibodies in cattle and buffalo serum. J. Virol. Methods 148: 218-225.
  11. Murugkar HV, Nagarajan S, Tosh C, Bhatia S, Venkatesh G, Jain R, Kumar S, Khandia R, Pandey M, Behera P, Tripathy S, Kulkarni DD, Dubey SC. (2008) H5N1 virus outbreaks in poultry in India. Vet Rec. 162 (8):255.
  12. Bhatia S., Sood R., Mishra, N., Pattnaik, B. and Pradhan H. K. (2007). Development and evaluation of a MAb based competitive-ELISA using helicase domain of NS3 protein for sero-diagnosis of bovine viral diarrhea in cattle and buffaloes. Res. Vet. Sci. 85 (1): 39-45.
  13. Tosh, C., H. V. Murugkar, S. Nagarajan, S. Bhatia, A. K. Pateriya, P. Behera, R. Jain, S. Kumar, R. Khandia, P. R. Vanamayya, S. C. Dubey (2007). Outbreak of avian influenza virus H5N1 in India. Vet Rec. 161 (8): 279.
  14. Mishra, N., Pattnaik, B., Vilcek, S., Patil, S.S., Jain, P., Swamy, N., Bhatia, S., and Pradhan, H.K. (2004) Genetic typing of bovine viral diarrhoea virus isolates from India. Vety. Microbiol. 104 (3-4): 207-12.

Project

  1. ICAR National Fellow Project on "Development and evaluation of neuraminidase DIVA marker vaccines against highly pathogenic H5N1 Avian Influenza viruses in Chickens".
  2. Development of an immuno-chromatographic test for the diagnosis of Avian Influenza virus infection in chicken (funded by NABARD).

Equal Opportunity for Persons with Disabilities

In accordance with the provisions of the Right of Persons with Disabilities Act, 2016 and Rules, the institute strives to provide opportunities and facilities to persons with disabilities to participate, perform and excel in their work on an equal basis in everyday life.